Potensi Antelmintika Ekstrak Bakteri Simbion Spons Laut Terhadap Trichostrongylidae (Nematoda) Parasit Domba. Muhammad Reza Faisal, Mujizat Kawaroe, Fadjar Satrija

ABSTRAK

Infeksi parasit trichostrongylidae (nematoda) yang menyerang domba memiliki resistansi terhadap antelmintika saat ini. Pemanfaatan bioaktif dari bakteri simbion spons memiliki potensi sebagai alternatif antelmintika yang alami terhadap infeksi parasit trichostrongylidae. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak bakteri simbion spons laut yang dapat menghasilkan golongan senyawa bioaktif sebagai antelmintika terhadap larva parasit trichostrongylidae pada domba. Isolat bakteri simbion spons yang dilabel S1 dan S2 diekstrak dengan pelarut metanol. Uji fitokimia dilakukan untuk menentukan karakterisasi golongan senyawa bioaktif yang berpotensi untuk menghambat migrasi larva. Konsentrasi yang digunakan untuk uji hambat migrasi larva trichostrongylidae domba, yaitu 25, 50, 100, 250, dan 500 µg/ml. Perlakuan kontrol positif dilakukan menggunakan larutan albendazol sementara kontrol negatif menggunakan larutan NaCl fisiologis. Kedua ekstrak memiliki kandungan toksisitas dalam membunuh larva Artemia salina di bawah konsentrasi <1000 µg/ml. Semakin tinggi konsentrasi ekstrak S1 dan S2 yang diberikan menyebabkan peningkatan hambatan migrasi larva. Kedua ekstrak memiliki kemampuan menghambat migrasi larva parasit domba dengan nilai LC50, yaitu 165,63 µg/ml (S1) dan 374,9 µg/ml (S2). Kemampuan ekstrak dalam menghambat migrasi larva disebabkan adanya kandungan golongan senyawa bioaktif yang dimiliki, yaitu triterpenoid pada kedua ekstrak dan golongan senyawa flavonoid pada ekstrak S1. Perlakuan kontrol positif dengan albendazol menunjukkan aktivitas penghambatan tinggi, yaitu 95,5% dari total larva yang diuji.

Kata kunci: antelmintika, bakteri, larva nematoda, spons

ABSTRACT

Trichostrongylidae (nematodes) parasitic infection of sheep were recently resistance to anthelmintics. Bioactive utilization of bacteria derived sponges had potential as anthelmintics alternative naturally against trichostrongylidae parasitic infections. The Aims of this study was to determine the activity of bacteria derived sponge extracts which produced anthelmintics bioactive compounds against sheep trichostrongylidae parasite. Bacteria derived sponges isolates which labeled S1 and S2 were extracted by methanol. Phytochemical test were conducted to determined characterization of bioactive compounds which potentially to inhibit larvae migration. Concentration which used to Larva Migration Inhibition Assay (LMIA) were 25, 50, 100, 250, and 500 µg/ml. Positive control treatment was used albendazole while negative control by physiological of NaCl. Both of extracts were contained toxicity to againts Artemia salina larvae which <1000 µg/ml concentration. The higher concentration of S1 and S2 extracts were affected to increase larvae migration. Both of extracts were potential to inhibit larvae migration which LC50 value were 165.63 µg/ml (S1) and 374.9 µg/ml (S2). The ability of extracts which inhibit larvae migration caused by bioactive compounds which contained triterpenoids in both of extracts then flavonoid compounds only by S1. Albendazole was showed a highest inhibitory activity which contained 95.5% of the total test nematode larvae.

Keywords: anthelmintics, bacteria, nematode larvae, sponge

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